输卵管上皮细胞共培养对小鼠早期胚胎发育阻滞的影响

doi:10.11843/j.issn.0366-6964.2013.07.023

摘要/Abstract

摘要：

研究输卵管上皮细胞共培养对小鼠早期胚胎发育的影响，探索输卵管上皮细胞共培养克服胚胎发育阻滞的机理。建立纯化的小鼠输卵管上皮细胞系，并与合子进行共培养，用DCHFDA测定胚胎内部的H₂O₂含量，以MTT法测定输卵管上皮细胞活力,用RT-PCR检测CAT及GPX的转录状况。输卵管上皮细胞共培养能够显著提高2-细胞早期、4-细胞期胚胎发育率（P＜0.05），囊胚率也有所提高（P＞0.05）。输卵管上皮细胞共培养能够显著降低2-细胞早期胚胎内部的H₂O₂含量（P＜0.05）。小鼠阻滞胚胎中有H₂O₂蓄积，输卵管上皮细胞共培养能够降低Ⅰ和Ⅱ型阻滞胚胎的比例（P＞0.05），显著地提高Ⅲ型阻滞胚胎的比例（P＜0.05）。当胚胎发育到2-细胞早期时共培养细胞活力上升（P＞0.05），抗氧化基因CAT及GPX转录量增加（P＞0.05）。以上结果表明，输卵管上皮细胞共培养通过细胞-细胞间的相互作用降低胚胎细胞内部的活性氧，克服胚胎发育阻滞，促进胚胎发育，提高胚胎质量。

Abstract:

This experiment was conducted to study the effect of oviduct epithelial cells co-culture on the development of early embryos, and explored the mechanisms of oviduct epithelial cells co-culture on overcoming the developmental arrest in mouse. Mouse oviduct epithelial cell line was purified and used to co-culture the zygotes, H₂O₂ contents in embryos were measured by DCHFDA, MTT was used to measure the activity of epithelial cells, RT-PCR was applied to test the transcriptions of CAT and GPX genes. Oviduct epithelial cells co-culture can significantly improve the early 2-cell and 4-cell rates (P＜0.05), the blastocyst rate was also increased (P＞0.05). Oviduct epithelial cells co-culture can decrease the H₂O₂content in early 2-cell embryos (P<0.05). Also there was a deposit of H₂O₂ in the arrested embryos, the rates of typeⅠand Ⅱarrested embryos were decreased（P>0.05），type Ⅲ was increased（P＜0.05）by oviduct epithelial cells co-culture. On the other side, the cellular activity was improved (P＞0.05) when embryos developed to early 2-cell phase, at the same time, transcriptions of CAT and GPX were increased（P>0.05）. Those results indicated that oviduct epithelial cell co-culture can improve the development of embryos by cell-cell contact to decrease the reactive oxygen species content in embryos, overcome developmental arrest and promote embryo development.

中图分类号:

S814.8

庄利利，吴俊波，胡德宝，蔡丽娟，张也，李钟淑，方南洙. 输卵管上皮细胞共培养对小鼠早期胚胎发育阻滞的影响[J]. 畜牧兽医学报, 2013, 44(7): 1160-1166.

ZHUANG Li-li, WU Jun-bo, HU De-bao, CAI Li-juan, ZHANG Ye, LI Zhong-shu, FANG Nan-zhu. Effect of Oviduct Epithelial Cells Co-culture on the Developmental Arrest of Early Embryos in Mouse[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1160-1166.

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